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Water Quality Testing III: Multiplexed PCR for Testing Water Bacteria 953
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Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required to determine whether harmful microbes are present. One such test uses PCR in multiplexed format. In this experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
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Kit includes: instructions, control DNA and primers, DNA ladder, nonpathogenic E. coli, B. subtilis, S. marcenscens, chelating agent, proteinase K, PCR beads, gel loading dye, agarose, buffer, FlashBlue™ stain.
You need: thermal cycler, waterbaths or heating blocks for PCR, waterbath, electrophoresis tank, power supply, micropipettors with tips, balance, microcentrifuge, microwave oven or hot plate, UV transilluminator, water & ice.
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