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Water Quality Testing II: PCR-Based Testing of Water Contaminants 952
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Now your students can use PCR to detect water pollution due to sewage contamination. In this experiment safe bacterial strains will be provided and dilutions will be made to determine the number of bacterial cells that are required to obtain a successful PCR result. As an extension to this experiment students will be able to test for water contamination in samples they provide.
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Kit includes: instructions, control DNA and primers, DNA ladder, E. coli control strain, chelating agent, proteinase K, PCR beads, gel loading dye, agarose, electrophoresis buffer, FlashBlue™ stain.
You need: micropipettes to measure between 5 and 50 µl (or 5,10, 30, 50 µl fixed volume minipipets), waterbath, microcentrifuge, thermal cycler, electrophoresis tank and power supply.
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