Water Quality Testing II: PCR Testing of Water Contaminants
Now your students can use PCR to detect water pollution due to sewage contamination. In this experiment, safe bacterial strains will be provided to simulate the detection of microbes in contaminated water. As an extension to this experiment, students will be able to test for water contamination in samples they provide.
Kit includes: instructions, E. coli control strain, control DNA and primers, DNA ladder, chelating agent, proteinase K, PCR EdvoBeads™, gel loading dye, agarose, electrophoresis buffer, InstaStain® Ethidium Bromide and FlashBlue™ stain, Ultrapure water.
All You need: micropipets to measure between 5 and 50 µl, tips, waterbath, microcentrifuge, thermal cycler, electrophoresis apparatus, power supply, microwave or hot plate, UV transilluminator.
Storage: Some Components Require Refrigerator and Freezer Storage
Set Up 60 min. PCR 120 min. or overnight Electrophoresis 45 min.