940 - Introduction to ELISA Reactions
Description
When the surface of a freshly cut plant is placed on a charged membrane, whole cells, proteins and nucleic acids bind to it. This technique, known as tissue printing, is simple and yields detailed information about cell identification and biomolecule localization within cells. This experiment utilizes a monoclonal antibody to detect
Brassica
phloem cells. Examples of
Brassica
family members include broccoli, brussel sprouts, and cauliflower. The membrane with the tissue print is sequentially treated with the primary monoclonal antibody that is specific for
Brassica
phloem cells, followed by the enzyme linked secondary antibody and the enzyme specific substrate. Phloem cells are clearly identified as bright red.
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940
$95
For 10 Sets of Membrane Prints
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