With this experiment, your students will demonstrate general enzyme principles using specific plant enzymes which have important functions in biotechnology. Students perform tissue prints of seeds to examine what happens during malting. An additional activity allows students to quantify the activity of the enzyme amylase.
Kit includes: instructions, 3 types of barley seeds, iodine solution/stain, reaction buffer, starch, amylase enzyme powder, 1 ml pipets, starch indicator paper, petri plates, graph paper template.
You need: single edge razor blades, 2 waterbaths, spectrophotometer, automatic micropipet with tips, test tubes, microscope or magnifying lens, hot plate, cutting board, forceps, test tube racks, pipet pumps, lab glassware, 1N HCl, distilled water, ice and ice bucket.