Scientists can directly manipulate an organism's genome to produce a phenotype using engineered genes called transgenes. In this experiment, students will use fluorescent microscopy and PCR to analyze C. elegans (nematodes) that have been engineered to express the Green Fluorescent Protein (GFP).
*Kit contains LIVE materials. Please request materials 1 week prior to lab*
Growing Bacteria - Overnight Plating Worms - 15 minutes Worm Growth - 3-4 Days Set Up - 30 minutes PCR - 2 hours or overnight Electroophoresis - 90 minutes
Kit includes: instructions, C. elegans, E. coli OP50 cells, S-Buffer, Ready-Pour NGM Agar, NGM Medium Salts, UltraSpec-Agarose™ powder, electrophoresis buffer (50x), 10x Gel Loading Solution, InstaStain® Ethidium Bromide, FlashBlue™ Liquid Stain, tubes, pipets, wax beads, petri dishes, counting chamber, microscope slides, sterile loops
All you need: covered box, fluorescent and dissecting microscopes, timers, waterbath, incubator (optional), thermal cycler or three waterbaths, electrophoresis apparatus, power supply, balance, microcentrifuge, UV transilluminator or white light visualization system, automatic micropipets with tips, microwave or hot plate, pipet pump, flasks or beakers, hot gloves, distilled or deionized water, ice buckets and ice.
Storage: Some Components Require Refrigerator & Freezer Storage