Caenorhabditis elegans is a soil nematode with great potential for educational research, partly because of its rapid (3-day) life cycle, small size (1.0-mm-long adult), and ease of laboratory growth cultivation. In this experiment, students will observe and compare the effects of heavy metals found in the environment on normal and mutant strains of Caenorhabditis elegans (C. elegans).
*Kit contains LIVE materials. Please request materials 1 week prior to lab*
Kit includes: instructions, C. elegans-normal, C. elegans-Toxicity mutant, petri dishes, NGM medium salts, ReadyPour NGM Agar, E. coli 0P50 Bactobeads™, cell counting chambers, buffer, pipets, sterile loops, tubes and heavy metal compounds.
All You need: ethanol, timers, microscopes, waterbath, covered box, incubator (optional).
Storage: Some Components Require Refrigerator Storage
Growing Bacteria - overnight Plating Worms - 15 min. Worm Growth - 3-4 days Toxicity Experiment - 45-60 min.