The integrity and stability of DNA is essential to life. However, everyday this molecule is under assault from environmental stressors like UV radiation, mutagenic chemicals, and even normal metabolic processes. In this guided inquiry lab students will use the cutting edge technology of QPCR to investigate and quantify DNA damage due to physical (UV radiation) or chemical (DNAseI) disruptions. By designing and performing the experiments students will master advanced analytical and technical skills as well as deepen their understanding of key molecular biology and medical concepts.
Kit includes: instructions, qPCR master mix with fluorescent dye, primer mix, DNA template, experimental DNA sample, Ultra-Pure water, DNAsdI, DNA standard marker, UltraSpec-Agarose™, 50X electrophoresis buffer, 10X gel loading solution, FlashBlue™ stain, InstaStain® Ethidium Bromide, microcentrifuge & qPCR tubes.
All you need: qPCR thermal cycler, microcentrifuge, micropipets: 5-50 µl, water bath, horizontal gel electrophoresis apparatus, DC power supply, UV transilluminator, microwave or hot plate, pipet pump, balance, hot gloves, laboratory gloves, beaker, distilled ice, & ice.
DNA Damage: 45 mins QPCR Setup: 45 mins QPCR: 90 mins Electrophoresis: 60 mins Staining: 5 mins to overnight Analysis: 45 mins