Quantitative PCR (qPCR) is a molecular technique that allows us to visualize the amplification of a specific DNA in real time; it also allows us to quantify the exact amount of the target DNA in the sample. To do this, a dye is added to the PCR sample that fluoresces when bound to double-stranded DNA. As such, any increase in fluorescence correlates to an increase in DNA in the sample. This allows scientists to precisely quantitate the amount of DNA in a sample as it is being amplified. In this experiment, students will explore the principles of qPCR by analyzing a dilution series. The resulting samples can also be analyzed by agarose gel electrophoresis.
**This kit must be used with a RT Thermal Cycler**