Discovering Quantitative PCR Amplification and Analysis
Quantitative PCR (qPCR also known as real time PCR) simultaneously amplifies and detects targeted DNA allowing scientists to discover the starting amount of a specific DNA sequence in an experimental sample. This powerful and precise technology has become a cornerstone of modern genetics research, biological monitoring, and medical diagnostics. For students, performing qPCR offers the challenge of performing a mathematically and technically advanced experiment, the opportunity to understand key molecular concepts through hands on learning, and an essential skill for future research work. In this specially adapted education qPCR experiment, students will quantify the DNA concentration of four experimental samples using a standard curve approach and then confirm the experiment’s specificity and accuracy through gel electrophoresis, melt curve analysis, and data analysis.
**This kit must be used with a RT Thermal Cycler**
Kit includes: instructions, qPCR Master Mix with Fluorescent Dye, Primer Mix, DNA Template, Experimental DNA Samples, Ultra-Pure Water, DNA Standard Marker, UltraSpec-AgaroseTM, 50X Electrophoresis Buffer, 10x Gel Loading Solution, FlashBlue™ Stain, InstaStain® Ethidium Bromide, Microcentrifuge & qPCR Tubes.
All you need: qPCR Thermal Cycler, Microcentrifuge, Micropipets: 5-50 μl, Horizontal Gel Electrophoresis Apparatus, DC Power Supply, UV Transilluminator or White Light Box, Microwave or Hot Plate, Pipet Pump, Balance, Hot Gloves, Laboratory Gloves, Beaker, Distilled Water, Ice.
Storage: Some Components Require Freezer Storage Upon Receipt.
QPCR Setup 45 mins QPCR 90 mins Single Amplification Confirmation by Electrophoresis 60 mins Staining 5 mins to overnight Analysis of Results 45 mins