In this experiment, students will gain an understanding of the traditional three-step Polymerase Chain Reaction (PCR). Using PCR and Agarose Gel Electrophoresis, they will analyze a small section of Lambda DNA in a time-saving two-step process.
DOWNLOAD SAMPLE INSTRUCTIONS
Groups size: For 10 lab groups Time required: PCR Setup - 10 minutes PCR - 30 minutes Electrophoresis - 30 minutes Staining - 5 minutes to overnight PRODUCT UPDATE: This kit includes the new LyphoTemplate™ and LyphoPrimer™. Update effective for all #372 kits purchased after December 20, 2016. Please read accompanying literature for your kit. Kit includes: Instructions, PCR EdvoBeads™, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoTemplate™ Lambda DNA, TE Buffer, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes. All you need: DNA Electrophoresis, Thermal Cycler, Micropipets: 5-50 µl, UV or Blue Light Transilluminator, (2) Water Baths (if performing experiment with a Thermal Cycler), White Light Box (optional), Microcentrifuge & Microwave or Hot plate. Storage: Some Components Require Freezer Storage Upon Receipt