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In this experiment, students will gain an understanding of the traditional three-step Polymerase Chain Reaction (PCR). Using PCR and Agarose Gel Electrophoresis, they will analyze a small section of Lambda DNA in a time-saving two-step process.
For 10 Lab Groups
Complete in 60 min.
PRODUCT UPDATE: This kit includes the new LyphoTemplate™ and LyphoPrimer™. Update effective for all #372 kits purchased after December 20, 2016. Please read accompanying literature for your kit.
Kit includes: instructions, PCR EdvoBeads™, DNA template & primers, DNA size ladder, ultrapure water, wax beads, gel loading dye, agarose, electrophoresis buffer, InstaStain® Ethidium Bromide and FlashBlue™ stain.
All you need: 5-50 µl adjustable micropipets, tips, thermal cycler, electrophoresis apparatus, power supply, microwave or hot plate, UV transilluminator.
Storage: Some Components Require Freezer Storage
Download Edvotek® PCR & Electrophoresis Troubleshooting Guide
Download EDVO-Kit #372 Safety Data Sheets (SDS)
EDVOTEK BLOG - Making the Polymerase Chain Reaction Easier with PCR EdvoBeads™
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