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In Real Time PCR, amplification is monitored while the reaction is ongoing and allows for a quantitative analysis. A fluorescent dye added to the PCR reaction, binds to the DNA as it is being amplified, and the resulting fluorescence is measured during the reaction. In this Real Time PCR experiment, the reaction will be monitored for product throughout the cycling steps without the use of agarose gel electrophoresis.
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Kit includes: instructions, PCR beads, DNA template and primers, Ultrapure water, wax beads, ethidium bromide, microtiter plates.
All you need: thermal cycler, automatic micropipet with tips, microcentrifuge, balance, UV transilluminator.
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