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Polymerase Chain Reaction (PCR) is commonly used to determine paternity as it is a very sensitive method for DNA analysis. Your students will gain an understanding of the principles behind this non-forensic use of DNA Fingerprinting using their own DNA! This kit provides three sets of primers to carry out the PCR amplification of Alu element (PV92) on chromosome 16, the VNTR locus (D1S80) on chromosome 1, or 2 mitochondrial genes. For 6 runs of each PCR reaction.
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Kit includes: instructions, proteinase K, PCR Beads, control and primer DNA, microtubes, chelating agent, agarose, DNA ladder, practice gel loading solution, gel loading dye, electrophoresis buffer, FlashBlue™ stain.
All you need: micropipettes to measure between 5 and 50 µl (or 5,10, 30, 50 µl fixed volume minipipets), waterbath, thermal cycler, electrophoresis tank and power supply.
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