Human PCR Toolbox™
Carry out three PCR experiments in your class at once! This kit provides three sets of primers to carry out the PCR amplification of Alu element (PV92) on chromosome 16, the VNTR locus (D1S80) on chromosome 1, and two regions of the mitochondrial gene. For 6 runs of each PCR reaction.
DOWNLOAD SAMPLE INSTRUCTIONS
Group size: For 25 students Time required:
Extraction - 50 minutes
PCR Setup - 10 minutes PCR - 2 hours Electrophoresis - 60 minutes Staining - 5 minutes to overnight PRODUCT UPDATE: PCR cycling change for PV92/Alu insert. Update effective for all #369 kits purchased after July 24th, 2014. Please read accompanying literature for your kit. Kit includes: instructions, PCR EdvoBeads™, Universal DNA Buffer, Mitochondrial LyphoPrimer™, Alu LyphoPrimer™ (PV92), VNTR LyphoPrimer™ (D1S80), EdvoQuick™ DNA ladder, Mitochondrial LyphoControl™, Alu LyphoControl™ (PV92), VNTR LyphoControl™ (D1S80), TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge and PCR tubes, disposable plastic cups, salt packets, & 15 mL conical tube. All you need: DNA Electrophoresis, Thermal Cycler, Micropipets: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), (2) Water Baths, Microcentrifuge & Microwave or Hot plate. Storage: Some Components Require Freezer Storage Upon Receipt.