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Drosophila Genotyping Using PCR 337
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Students will learn about DNA polymorphisms by amplifying DNA regions that vary between wild & mutant Drosophila. Amplified DNA from wild-type and white-eyed flies are separated by agarose gel electrophoresis and analyzed.
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Kit includes: instructions, PCR beads, primers, DNA extraction buffer, 200 bp ladder, Proteinase K, Drosophila (wild type and white eye), agarose, electrophoresis buffer, InstaStain® Ethidium Bromide and FlashBlue™ stain.
All you need: thermal cycler, electrophoresis apparatus and power supply, automatic micropipet with tips, microwave or hot plate, waterbath, UV transilluminator, microcentrifuge.
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