Drosophila Genotyping Using PCR
Students will learn about DNA polymorphisms by amplifying DNA regions that vary between wild & mutant Drosophila. Amplified DNA from wild-type and white-eyed flies are separated by agarose gel electrophoresis and analyzed.
See Below for Time Requirements
Kit includes: Instructions, PCR EdvoBeads™, Primer Mix Concentrate, 200 Base Pair Ladder, Control DNA Concentrate, TE Buffer, Proteinase K, Potassium Acetate, DNA Extraction Buffer, Wild & White Drosophila, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain® Ethidium Bromide, FlashBlue™ Liquid Stain, Conical, Microcentrifuge, & PCR Tubes, Transfer Pipets, & Wax Beads. All you need: DNA Electrophoresis, Thermal Cycler, Micropipets: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), Water Bath, Microcentrifuge & Microwave or Hot plate. Storage: Some Components Require Freezer Storage Upon Receipt.