Drosophila Genotyping Using PCR
Students will learn about DNA polymorphisms by amplifying DNA regions that vary between wild & mutant Drosophila. Amplified DNA from wild-type and white-eyed flies are separated by agarose gel electrophoresis and analyzed.
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Group size: For 10 lab groups Time required: Extraction - 50 minutes PCR Setup - 10 minutes PCR - 2 hours Electrophoresis - 60 minutes Staining - 5 minutes to overnight Kit includes: Instructions, PCR EdvoBeads™, Primer Mix Concentrate, 200 Base Pair Ladder, Control DNA Concentrate, TE Buffer, Proteinase K, Potassium Acetate, DNA Extraction Buffer, Wild & White Drosophila, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain® Ethidium Bromide, FlashBlue™ Liquid Stain, Conical, Microcentrifuge, & PCR Tubes, Transfer Pipets, & Wax Beads. All you need: DNA Electrophoresis, Thermal Cycler, Micropipets: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), Water Bath, Microcentrifuge & Microwave or Hot plate. Storage: Some Components Require Freezer Storage Upon Receipt.