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Determining Brassica QuickPlant™ Genetics Using PCR 336
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Your students will see for themselves the relationship between genotype and phenotype by performing PCR using DNA extracted from Edvotek® Quick-Plants™. Unlike the wild type QuickPlants™, the glabra mutant lacks trichomes (single-celled hairs) on its leaves. Using PCR, your students will compare a region of DNA that differs between the glabra mutant and the wild type plants, so they will see this variation at the DNA level.
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Kit includes: instructions, QuickPlant™ seeds, potting soil pellets and pots, PCR beads, microtubes, primers, DNA extraction buffer, plant homogenization pestles with tubes, agarose, electrophoresis buffer, DNA ladder, InstaStain® Ethidium Bromide FlashBlue™ stain.
All you need: micropipettes to measure between 5 and 50 µl, tips, waterbath, thermal cycler, electrophoresis apparatus, power supply, microwave or hot plate, UV transilluminator.
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