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RT-PCR: The Molecular Biology of HIV Replication 335
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A specific mRNA is reverse transcribed to double-stranded DNA. This DNA product is then amplified by PCR. This reaction demonstrates the mode of replication of HIV, which contains reverse transcriptase. This experiment is the first introduction of a commercial RNA experiment for the classroom laboratory.
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Kit includes: instructions, RNA Template, primer Mix, RT-PCR reaction beads, RNase-free water, standard DNA markers, agarose, electrophoresis buffer, InstaStain® Ethidium Bromide, gel loading solution.
All You need: thermal cycler, electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave or hot plate, UV transilluminator, pipet pump or bulb, lab glassware, distilled or deionized water.
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