Reverse Transcription PCR (RT-PCR): The Molecular Biology of HIV Replication
A specific mRNA is reverse transcribed to double-stranded DNA. This DNA product is then amplified by PCR. This reaction demonstrates the mode of replication of HIV, which contains reverse transcriptase. This experiment is the first introduction of a commercial RNA experiment for the classroom laboratory.
* Requires wet ice shipment for next day delivery (by 3:00 pm in most areas)
All You need: thermal cycler, electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave or hot plate, UV transilluminator, pipet pump or bulb, lab glassware, distilled or deionized water.
Storage: Some Components Require Freezer Storage
PCR Setup 10 mins PCR 3 hours Electrophoresis 60 mins Staining 5 mins