Reverse Transcription PCR (RT-PCR): The Molecular Biology of HIV Replication

SKU: 335
$249.00

ABOUT THIS PRODUCT:


A specific mRNA is reverse transcribed to double-stranded DNA. This DNA product is then amplified by PCR. This reaction demonstrates the mode of replication of HIV, which contains reverse transcriptase. This experiment is the first introduction of a commercial RNA experiment for the classroom laboratory.

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 6 lab groups

Time Required:

PCR Setup - 10 minutes
PCR - 3 hours
Electrophoresis - 60 minutes
Staining - 5 minutes

Kit Includes:

Instructions, RNA LyphoTemplate™, LyphoPrimer™ mix, tubes with RT-PCR EdvoBeads™, RNAse-free water, EdvoQuick™ DNA Ladder, UltraSpec-Agarose™, 50X Electrophoresis Buffer, 10X Gel Loading Solution, SYBR® Safe stain, microcentrifuge & PCR tubes.

All You Need:

DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, (3) Water Baths (if performing experiment without a thermal cycler), Microcentrifuge & Microwave or Hot plate.

Storage:

Some Components Require Freezer Storage Upon Receipt

Resources

Quick Guides:

Troubleshooting Guides:

Safety Data Sheets:

Instructional Videos:

Replenisher

Group Size:

For 6 lab groups

Time Required:

PCR Setup - 10 minutes
PCR - 3 hours
Electrophoresis - 60 minutes
Staining - 5 minutes

Kit Includes:

Instructions, RNA LyphoTemplate™, LyphoPrimer™ mix, tubes with RT-PCR EdvoBeads™, RNAse-free water, EdvoQuick™ DNA Ladder, UltraSpec-Agarose™, 50X Electrophoresis Buffer, 10X Gel Loading Solution, SYBR® Safe stain, microcentrifuge & PCR tubes.

All You Need:

DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, (3) Water Baths (if performing experiment without a thermal cycler), Microcentrifuge & Microwave or Hot plate.

Storage:

Some Components Require Freezer Storage Upon Receipt

Quick Guides:

Troubleshooting Guides:

Safety Data Sheets:

Instructional Videos:

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