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RT-PCR: A Model for the Molecular Biology of HIV Replication 335
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A specific mRNA is reverse transcribed to double-stranded DNA. This DNA product is then amplified by PCR. This reaction demonstrates the mode of replication of HIV, which contains reverse transcriptase. This experiment is the first introduction of a commercial RNA experiment for the classroom laboratory.
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Kit includes: instructions, RNA template, primer mix, RT-PCR reaction beads, RNase-Free water, standard DNA markers, agarose, electrophoresis buffer, InstaStain® Ethidium Bromide, gel loading solution.
You need: thermal cycler, horizontal gel electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave oven or hot plate, waterbath, UV transilluminator, pipet pump or bulb, lab glassware, distilled water.
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