Your students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype! They can then compare their class results with others around the world over the internet.
PRODUCT UPDATE: PCR cycling change for PV92/Alu insert. Update effective for all #333 kits purchased after July 24th, 2014. Please read accompanying literature for your kit.
Kit includes: instructions, proteinase K, PCR EdvoBeads™, control DNA and primers, microtubes, agarose, DNA ladder, practice gel loading solution, gel loading dye, buffer, InstaStain® Ethidium Bromide and FlashBlue™ stain.
All you need: micropipets to measure between 5 and 50 µl, tips, waterbath, thermal cycler, electrophoresis apparatus, power supply, microwave or hot plate, UV transilluminator.
Storage: Some Components Require Freezer Storage
Extraction 50 mins PCR Setup 10 mins PCR 90 mins Electrophoresis 60 mins Staining 5 mins to overnight