Alu Human DNA Typing Using PCR
Your students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype! They can then compare their class results with others around the world over the internet.
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Group size: For 25 students Time required: Extraction - 50 minutes PCR Setup - 10 minutes PCR - 90 minutes Electrophoresis - 60 minutes Staining - 5 minutes to overnight PRODUCT UPDATE: PCR cycling change for PV92/Alu insert. Update effective for all #333 kits purchased after July 24th, 2014. Please read accompanying literature for your kit. Kit includes: Instructions, PCR EdvoBeads™ PLUS, PV92 LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™, Universal DNA Buffer, TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR®Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & a 15 mL conical tube. All you need: DNA Electrophoresis, Thermal Cycler, Micropipets: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), (2) Water Baths, Microcentrifuge & Microwave or Hot plate. Storage: Some Components Require Freezer Storage Upon Receipt