331 - Cloning of a PCR Amplified Gene

Cloning of a PCR Amplified Gene
Cat. #331

Teach your students about cloning with this exciting and exclusive lab! An antibiotic gene is amplified using PCR and then the size is determined by using DNA standard markers and agarose gel electrophoresis. T4 DNA Ligase is used to insert the antibiotic gene into a plasmid vector and the resulting recombinant DNA ("clone") is used to transform E. coli LyphoCells™. The transformed cells are plated and transformants are counted to determine transformation efficiency.

Kit includes: instructions, biologicals, buffers and reagents for PCR, ligation and transformation, ReadyPour™ Luria Broth agar, DNA size ladder, wax beads, agarose, electrophoresis buffer and gel stain.
All you need: EdvoCycler™, two waterbaths, incubation oven, electrophoresis tank, power supply, variable micropipet and tips, UV transilluminator, balance and microwave or hot plate.
For 5 Lab Groups
Setup 30 minutes, PCR 2 hours or overnight, Electrophoresis 45 minutes

Want to learn more? Download a PDF of the instructions or email us with questions.

Secure Ordering

Catalog # Price Quantity

331 $260

For 5 Lab Groups with InstaStain® Ethidium Bromide

625 $95

Extra PCR Beads (for 25 reactions)

642 $10

Extra 0.2 ml Thin-Walled PCR Microtest Tubes (Package of 100)

You may also place an order by phone, fax, email or regular mail:

E D V O T E K® -- The Biotechnology Education Company®
tel 800.338.6835 | fax 301.340.0582 | info@edvotek.com
Post Office Box 341232; Bethesda, MD 20827-1232 USA