317 - Western Blot Analysis (Polyacrylamide-based)

Western Blot Analysis (Polyacrylamide-based)
Cat. #317

Show your students how the Western blot identifies proteins based on both antibody reactions and antigens. Proteins are separated on a denaturing SDS polyacrylamide gel and are transferred (blotted) to a nylon membrane. The membrane is then exposed sequentially to solutions containing primary antibody, followed by a secondary antibody coupled to an enzyme. The membrane is soaked in a substrate solution to develop the color reaction, which results in identifying the antigen as a band. The molecular weights of the visible bands are measured using prestained protein markers of known molecular weight. This experiment does not require an electrotransfer apparatus.

Kit includes: instructions, negative control, BSA - high and low concentration, anti-BSA protein antibody, secondary antibody conjugate, hydrogen peroxide, peroxide co-substrate, blocking buffer, powdered milk, PBS buffer, prestained protein standard marker, membrane and filter paper and electrophoresis buffer.
All you need: 3 polyacrylamide gels (12%), MV10 vertical gel electrophoresis apparatus, power supply, automatic micropipet with fine tips, trays for blot transfer, lab glassware, plastic wrap, metric rulers, methanol and glacial acetic acid.
For 6 Blots
Complete in 60 minutes, Blot Overnight, Detection 150 minutes

Want to learn more? Download a PDF of the instructions or email us with questions.

Secure Ordering

Catalog # Price Quantity

317 $199

For 6 Blots

651 $39

Pre-cast Polyacrylamide Gels (3)

652 $74

Pre-cast Polyacrylamide Gels (6)

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E D V O T E K® -- The Biotechnology Education Company®
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