316 - In Search of the Cholesterol Gene

In Search of the Cholesterol Gene
Cat. #316

Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor for both conditions. The genetic disease familial hypercholesterolemia (FH) causes an increase in blood levels of the "bad" form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students will analyze a simulated genetic screening for a disease.

Kit includes: instructions, cholesterol standard solution, standard DNA markers, control samples, simulated patient serum samples and DNA samples, cholesterol oxidase enzyme, potassium iodide, acidification solution, color enhancer & color developer, agarose, electrophoresis buffer and stain.
All you need: electrophoresis tank, power supply, variable micropipet and tips, balance and microwave or hot plate, incubation oven or waterbath, spectrophotometer and UV transilluminator.
For 10 Lab Groups
Gel Preparation 30 minutes, Electrophoresis 45 minutes, Staining 2 minutes, Cholesterol Assay 60 minutes.

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Secure Ordering

Catalog # Price Quantity

316 $149

For 10 Lab Groups with InstaStain® Ethidium Bromide

651 $39

Pre-cast Polyacrylamide Gels (3)

652 $74

Pre-cast Polyacrylamide Gels (6)

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E D V O T E K® -- The Biotechnology Education Company®
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Post Office Box 341232; Bethesda, MD 20827-1232 USA