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In Search of the Sickle Cell Gene by Southern Blot 315
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Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane, the method allows you to analyze and identify the DNA bands on a gel precisely. Your students will use Southern blotting to find a point mutation in the hemoglobin gene indicating Sickle Cell Anemia.
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Kit includes: instructions, Ready-to-Load™ DNA samples, agarose, electrophoresis buffer, nylon membranes, filter paper, blot stain.
All you need: electrophoresis apparatus, power supply, microwave or hot plate, waterbath and 80ºC incubation oven.
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