In Search of the Sickle Cell Gene by Southern Blot
Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane, the method allows you to analyze and identify the DNA bands on a gel precisely. Your students will use Southern blotting to find a point mutation in the hemoglobin gene indicating Sickle Cell Anemia.
For 5 Lab Groups
Electro. (45 min) Blot (Overnight) Stain/DeStain (10 min)