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In Search of the Sickle Cell Gene by Southern Blot
Cat. #315

Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane, the method allows you to analyze and identify the DNA bands on a gel precisely. Your students will use Southern blotting to find a point mutation in the hemoglobin gene indicating Sickle Cell Anemia.

For 5 Lab Groups

Electro. (45 min) Blot (Overnight) Stain/DeStain (10 min)

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Kit includes: instructions, Ready-to-Load™ DNA samples, agarose, electrophoresis buffer, nylon membranes, filter paper, blot stain.

All you need: electrophoresis apparatus, power supply, microwave or hot plate, waterbath and 80ºC incubation oven.

Storage: Some Components Require Freezer Storage

Download EDVO-Kit #315 Safety Data Sheets (SDS)
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  • EXPERIMENTS
  • Discovering Electrophoresis
  • DNA Electrophoresis (Ready-to-Load™)
  • Advanced DNA Applications
  • Polymerase Chain Reaction (PCR)
  • Genetic Engineering & Transformation
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  • Environmental Science
  • Proteins, Enzymes & Chromatography
  • Introduction to Biotechnology
  • Advanced Placement Biology
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  • PCR Equipment
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