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Exploring Biotechnology with Green Fluorescent Protein (GFP)
Cat. #303



Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the green fluorescent protein (GFP). The transformed cells are then grown and the GFP is purified by column chromatography. Finally, the purity of the protein fractions are analyzed by SDS polyacrylamide electrophoresis.



Kit includes: instructions, transformation cells, plasmid DNA for GFP, IPIG, ampicillin antibiotic, calcium chloride, ReadyPour™ luria broth agar, luria broth media for recovery, petri plates, pipets, calibrated transfer pipets, inoculating loops, microtest tubes with attached caps,, toothpicks, dry matrix for columns, chromatography columns, green and blue fluorescent protein extracts, elution buffer, protein molecular weight standards, protein denaturation solution, glycerol solution, Tris-Glycine-SDS buffer, stains.

All you need: incubation oven, two waterbaths, microwave or hot plate, automatic micropipet and tips, pipet pumps or bulbs, ice, long wave UV light, ring stand and clamps, lab glassware, ice, vertical gel electrophoresis apparatus and power supply, 3 Polyacrylamide Gels (12%), plastic trays or large weigh boats for optional staining & destaining, glacial acetic acid, methanol.

  For 6 experiments with 4 modules each

  Transformation 45 minutes, Plating 5 minutes, Incubation overnight, Transformation effc 15 minutes, Packing and running the column 45 minutes, Optional electrophoresis 60 minutes, Staining/destaining 30-120 minutes.


Want to learn more? Download a PDF of the instructions or email us with questions.

  Secure Ordering


Catalog #PriceQuantity

303$150
For 6 experiments with 4 modules each



You may also place an order by phone, fax, email or regular mail:

E D V O T E K® -- The Biotechnology Education Company®
tel 800.338.6835 | fax 301.340.0582 |
info@edvotek.com
Post Office Box 341232; Bethesda, MD 20827-1232 USA








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