All you need: incubation oven, two waterbaths, microwave or hot plate, automatic micropipet and tips, pipet pumps or bulbs, ice, long wave UV light, ring stand and clamps, lab glassware, ice, vertical gel electrophoresis apparatus and power supply, 3 Polyacrylamide Gels (12%), plastic trays or large weigh boats for optional staining & destaining, glacial acetic acid, methanol.
For 6 experiments with 4 modules each
Transformation 45 minutes, Plating 5 minutes, Incubation overnight, Transformation effc 15 minutes, Packing and running the column 45 minutes, Optional electrophoresis 60 minutes, Staining/destaining 30-120 minutes.
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