302 - Purification of the Restriction Enzyme Eco RI

Purification of the Restriction Enzyme Eco RI
Cat. #302

In this experiment, students actually purify the restriction enzyme, Eco RI! This procedure utilizes an ion exchange chromatography step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for college level courses.

Kit includes: instructions, ion exchange matrix, chromatography columns, E. coli cell extract, equilibration & elution buffer, Lambda DNA, Lambda/Eco RI Marker, KCl, glycerol, dilution & reaction buffers, gel loading solution, agarose, electrophoresis buffer and stain.
All you need: horizontal gel electrophoresis apparatus, power supply, UV visualization system, waterbath, microcentrifuge, microwave or hot plate, UV spectrophotometer & cuvettes, automatic micropipet with tips, ring stands & clamps, 10 ml pipets, lab glassware, ice and ice buckets.
For 5 Lab Groups
Packing column 45 minutes, Restriction analysis A 35 minutes, Restriction analysis B 50 minutes, Gel Preparation 30 minutes, Electrophoresis 30 minutes and Staining/Destaining 2 minutes.

Want to learn more? Download a PDF of the instructions or email us with questions.

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302 $159

For 5 Purifications

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E D V O T E K® -- The Biotechnology Education Company®
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