301 - Construction & Cloning of a DNA Recombinant

Construction & Cloning of a DNA Recombinant
Cat. #301

Cloning is frequently performed to study gene structure, function, and to enhance gene expression. This experiment is divided into five modules. Clones are constructed by ligation of a vector and a fragment insert. The constructs are then transformed into competent cells and the cells are grown and selected for resistance. Plasmid DNA is then isolated from the transformants, cleaved with restriction enzymes, and analyzed by agarose gel electrophoresis. Recommended for college level courses. This experiment requires wet ice shipment for next day delivery (by 3:00 pm in most areas).

Kit includes: instructions, enzymes, plasmid DNA, restriction enzyme dilution buffer, enzyme grade water, standard DNA fragments, restriction enzyme reaction buffer, gel loading solution, agarose powder, electrophoresis buffer, stains and calibrated pipet.
All you need: electrophoresis tank and power supply, automatic micropipet with tips, balance, microwave or hot plate, waterbath, large weigh boats for staining, UV transilluminator, floating racks for microtest tubes, pipet pump or bulb, 5 or 10 ml pipets, laboratory glassware, metric rulers, distilled water and ice.
For 5 Lab Groups
Enzyme Digestion 30 minutes, Gel Preparation 30 minutes, Electrophoresis 45 minutes, Staining 15 minutes and Destaining 20 minutes

Want to learn more? Download a PDF of the instructions or email us with questions.

Secure Ordering

Catalog # Price Quantity

301 $259

For 5 Plasmid Constructs and Analyses with InstaStain® Ethidium Bromide

You may also place an order by phone, fax, email or regular mail:

E D V O T E K® -- The Biotechnology Education Company®
tel 800.338.6835 | fax 301.340.0582 | info@edvotek.com
Post Office Box 341232; Bethesda, MD 20827-1232 USA