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Construction and Cloning of a DNA Recombinant
Cat. #301

Cloning is frequently performed to study gene structure and function, and to enhance gene expression. This experiment is divided into five modules. Clones are constructed by ligation of a vector and a fragment insert. The constructs are then transformed into competent cells and the cells are grown and selected for resistance. Plasmid DNA is then isolated from the transformants, cleaved with restriction enzymes, and analyzed by agarose gel electrophoresis. Recommended for college level courses.

For 5 Plasmid Constructs & Analyses

Complete 5 modules in approximately 5 hrs.

Download Sample Instructions
Description
Features
Resources

PRODUCT UPDATE: Storage conditions for some components have changed as of 02/17/17. Be sure to download the most recent version of the literature before performing the experiment.

Kit includes: instructions, BactoBeads™, enzymes, plasmid DNA, restriction enzyme dilution buffer, enzyme grade water, standard DNA fragments, restriction enzyme reaction buffer, gel loading solution, agarose powder, electrophoresis buffer, stains, calibrated pipet

You need: electrophoresis apparatus and power supply, automatic micropipet with tips, balance, microwave or hot plate, waterbath, large weigh boats for staining, UV transilluminator, floating racks for microtest tubes, pipet pump or bulb, 5 or 10 ml pipets, laboratory glassware, metric rulers, distilled water, ice.

Storage: Some Components Require Freezer & Refrigerator Storage

Download Edvotek® Quick Guide: Transformation

Download Edvotek® Transformation Troubleshooting Guide

Download EDVO-Kit #301 Safety Data Sheets (SDS)

EDVOTEK BLOG – DNA Ligase – Building a Bridge with DNA

EDVOTEK BLOG – Five Ways to Improve Your Transformation Experiments

EDVOTEK BLOG – Sequencing Tardigrade DNA Reveals Unexpected Results

Watch Videos
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<iframe width="329" height="185" src="//www.youtube.com/embed/DPACU2boNO0?list=PLdpmHMO6NC2ziJIs3i3uZrWe6zHEV5vpL" frameborder="0" allowfullscreen></iframe>
<iframe width="329" height="185" src="//www.youtube.com/embed/lJB2hmOxkzE?list=PLdpmHMO6NC2ziJIs3i3uZrWe6zHEV5vpL" frameborder="0" allowfullscreen></iframe>
  • Transformation " >
  • Preparation of Bacterial Source Plates " >
  • Pouring Agar Plates " >
  • Preparing the Agarose Gel Tray " >
  • Performing Electrophoresis
  • Staining Agarose Gels Using InstaStain® Ethidium Bromide
  • Visualizing DNA Using SYBR® Safe Stain
  • Transformation
  • Preparation of Bacterial Source Plates
  • Pouring Agar Plates
  • Preparing the Agarose Gel Tray
  • Performing Electrophoresis
  • Staining Agarose Gels Using InstaStain® Ethidium Bromide
  • Visualizing DNA Using SYBR® Safe Stain
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  • EXPERIMENTS
  • Discovering Electrophoresis
  • DNA Electrophoresis (Ready-to-Load™)
  • Advanced DNA Applications
  • Polymerase Chain Reaction (PCR)
  • Genetic Engineering & Transformation
  • Forensics
  • Biomedical Sciences & Immunology
  • Environmental Science
  • Proteins, Enzymes & Chromatography
  • Introduction to Biotechnology
  • Advanced Placement Biology
  • Reagents, Biologicals & Supplies
  • EQUIPMENT
  • DNA Electrophoresis
  • PCR Equipment
  • Protein Electrophoresis
  • Pipets & Liquid Handling
  • Visualization & Analysis
  • Water Baths & Incubation
  • Microcentrifuges
  • LabStation™ Equipment Packages
  • Biotech Bargains
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