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Construction and Cloning of a DNA Recombinant

SKU: 301
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$449.00

ABOUT THIS PRODUCT:


Cloning is frequently performed to study gene structure and function, and to enhance gene expression. This experiment is divided into five modules. Clones are constructed by ligation of a vector and a fragment insert. The constructs are then transformed into competent cells and the cells are grown and selected for resistance. Plasmid DNA is then isolated from the transformants, cleaved with restriction enzymes, and analyzed by agarose gel electrophoresis. Recommended for college-level courses.

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Details

PRODUCT UPDATE:

Storage conditions for some components have changed as of 02/17/17. Be sure to download the most recent version of the literature before performing the experiment.


Group Size:

For 5 plasmid constructs & analyses


Time Required:

Complete 5 modules in approximately 5 hours


Kit Includes:

Instructions, BactoBeads™, enzymes, plasmid DNA, restriction enzyme dilution buffer, enzyme grade water, standard DNA fragments, restriction enzyme reaction buffer, gel loading solution, agarose powder, electrophoresis buffer, SYBR® Safe DNA stain, calibrated pipet


You Need:

Electrophoresis apparatus and power supply, automatic micropipette with tips, balance, microwave or hot plate, water bath, large weigh boats for staining, UV transilluminator or blue light visualization system, floating racks for microtest tubes, pipet pump or bulb, 5 or 10 ml pipets, laboratory glassware, metric rulers, distilled water, ice.


Storage:

Some Components Require Freezer & Refrigerator Storage

Resources

Instructional Videos:

EdvoTech Tips:

Edvotek Informational Video:

Additional Resources:

Edvotek® YouTube Live Stream - AMA EDVO-Kit #301 Construction & Cloning of a DNA Recombinant - In the lab, we can use molecular tools to cut and paste pieces of DNA from different organisms, allowing us to study gene function or even transform bacteria into living factories! These powerful molecular cloning techniques are used daily in research labs around the world, but simple enough to be performed in your classroom laboratory. In this live stream, Dr. Danielle Snowflack discusses each module of experiment #301, highlights potential pitfalls, and provides tricks for experimental success!

Miscellaneous:

Replenisher

PRODUCT UPDATE:

Storage conditions for some components have changed as of 02/17/17. Be sure to download the most recent version of the literature before performing the experiment.


Group Size:

For 5 plasmid constructs & analyses


Time Required:

Complete 5 modules in approximately 5 hours


Kit Includes:

Instructions, BactoBeads™, enzymes, plasmid DNA, restriction enzyme dilution buffer, enzyme grade water, standard DNA fragments, restriction enzyme reaction buffer, gel loading solution, agarose powder, electrophoresis buffer, SYBR® Safe DNA stain, calibrated pipet


You Need:

Electrophoresis apparatus and power supply, automatic micropipette with tips, balance, microwave or hot plate, water bath, large weigh boats for staining, UV transilluminator or blue light visualization system, floating racks for microtest tubes, pipet pump or bulb, 5 or 10 ml pipets, laboratory glassware, metric rulers, distilled water, ice.


Storage:

Some Components Require Freezer & Refrigerator Storage

Instructional Videos:

EdvoTech Tips:

Edvotek Informational Video:

Additional Resources:

Edvotek® YouTube Live Stream - AMA EDVO-Kit #301 Construction & Cloning of a DNA Recombinant - In the lab, we can use molecular tools to cut and paste pieces of DNA from different organisms, allowing us to study gene function or even transform bacteria into living factories! These powerful molecular cloning techniques are used daily in research labs around the world, but simple enough to be performed in your classroom laboratory. In this live stream, Dr. Danielle Snowflack discusses each module of experiment #301, highlights potential pitfalls, and provides tricks for experimental success!

Miscellaneous: