300 - Blue/White Cloning of a DNA Fragment and Assay of ß-galactosidase

Blue/White Cloning of a DNA Fragment and Assay of ß-galactosidase
Cat. #300

When DNA is subcloned in the pUC polylinker region, ß-galactosidase production is interrupted, resulting in the inability of cells to hydrolyze X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA Ligase. Following the ligation to synthesize the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. ß-galactosidase activity is assayed from blue and white bacterial cells.

Kit includes: instructions, Linearized pUC plasmid & DNA fragment, T4 Ligase, Bacterial LyphoCells™ for transformation, reconstitution buffer, X-Gal in solvent, IPTG, calcium chloride, antibiotic, ReadyPour™ Luria Broth Agar, Luria broth media for recovery, growth media, assay components and plastic supplies.
All you need: incubation oven, two waterbaths, shaking incubator or shaking waterbath, microwave or hot plate, automatic micropipet and tips, spectrophotometer, balance, centrifuge, microcentrifuge, glassware and cuvettes, distilled water and ice.
For 5 Lab Groups
Ligation 60 minutes, Transformation 45 minutes, Plating 5 minutes, Incubation overnight & Transformation 15 minutes.

Want to learn more? Download a PDF of the instructions or email us with questions.

Secure Ordering

Catalog # Price Quantity

300 $159

For 5 Lab Groups

You may also place an order by phone, fax, email or regular mail:

E D V O T E K® -- The Biotechnology Education Company®
tel 800.338.6835 | fax 301.340.0582 | info@edvotek.com
Post Office Box 341232; Bethesda, MD 20827-1232 USA