LAB 12: Dissolved Oxygen and Aquatic Primary Productivity
Dissolved oxygen levels are used to monitor the health and productivity of aquatic ecosystems. In this kit, students familiarize themselves with the Winkler method by examining the relationship between temperature and dissolved oxygen concentrations. Using local water samples to examine primary productivity at different water depths, students can then observe the beginning stages of eutrophication.
Kit includes: instructions, sodium iodide, sodium hydroxide, manganese sulfate, starch solution, sodium thiosulfate, nitrogen enrichment solution, phosphorous enrichment reagents, transfer pipets, microcentrifuge tubes, and 15 ml conical tubes.
All you need: tap water, local water samples or Chlorella spiked water, light source, burettes, flasks or beakers (at least 500 ml), graduated cylinder (at least 200 ml), distilled water, thermometers, screens, rubber bands, goggles and gloves, sulfuric acid, concentrated (98%+ or 18 mol/L+), acid spill clean up material, glass pipet, pipet pump, filter paper (#1 Whatman), aluminum foil, waterproof markers, linear graph paper or computer with spreadsheet program, microscope, microscope slides, and cover slips (optional)