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LAB 4: Plant Pigment Chromatography and Photosynthesis 284
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Chromatographic separation of carotene, xanthophyll and chlorophylls are performed by thin layer chromatography. The reformulated solvent system minimizes toxicity. Key reagents are included to demonstrate photosynthesis.
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Kit includes: instructions, chloroplast extraction & dilution buffers, reducing reagent, DCIP, standard pigments, chromatography solvent, Sarkosyl, thin layer cellulose chromatography plate, microliter capillary pipets, tubes and transfer pipets.
All you need: visible wavelength spectrophotometer, clinical benchtop centrifuge, blender, flood light, calculator, timer, test tubes & racks, beakers, 1 & 5 ml pipets, pipet pumps, metric rulers, cheesecloth, 95-100% isopropanol, distilled water, fresh spinach, ice.
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