276 - Clinical Diagnostic Dot Blot

Clinical Diagnostic Dot Blot
Cat. #276

The dot blot (immunoblot) technique is used to determine the presence of an antigen. Clinical diagnostic kits employ the principles of the dot blot. In this experiment, antigens are absorbed to a membrane that is then treated with an antigen-specific antibody solution and then a secondary antibody conjugated to an enzyme. The enzyme-substrate reaction generates a color product that precipitates onto the membrane, indicating a positive reaction. No human serum is used in this experiment.

Kit includes: instructions, antigen & antibodies, various reagents and buffers, instant nonfat dry milk, hydrogen peroxide, nylon membranes and petri dishes.
All you need: electrophoresis tank, power supply, variable micropipet and tips, balance and microwave or hot plate.
For 10 Lab Groups
Setup in 45 minutes, Experiment 90 - 120 minutes

Want to learn more? Download a PDF of the instructions or email us with questions.

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Catalog # Price Quantity

276 $99

For 10 Lab Groups

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E D V O T E K® -- The Biotechnology Education Company®
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