AIDS Kit II: Simulation of HIV Detection by Western Blot
The second assay used to confirm a positive HIV ELISA result is the Western Blot. Students separate protein samples from hypothetical patients on agarose gels, transfer the samples to a membrane and detect the simulated HIV proteins. This kit is an introductory level experiment. For a comprehensive advanced course, we recommend Cat. #317.
Time required: Electrophoresis - 45 minutes | Blot - overnight | Detection - 25 minutes
Kit includes: instructions, samples, standard molecular weight markers, protein agarose, various buffers and reagents, PVDF membrane, filter paper, stain, 1 ml pipet, 100 ml graduated cylinder.
All you need: electrophoresis apparatus, power supply, automatic micropipets with tips, microwave or hot plate, incubation oven, shaker platform, lab glassware, small plastic trays, microtest tubes, pipet pumps or bulbs, metric rulers, distilled water, methanol, glacial acetic acid.
Storage: Some Components Require Refrigerator Storage