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Transformation of E. coli with pGAL™ (Blue Colony) 221
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In this experiment, your students can see a blue color change in transformed cells due to the switching on of a gene. The pGAL™ plasmid gives them a blue color due to the production of the ß-galactosidase protein by the lacZ gene. IPTG is not required in this experiment since pGAL™ contains the complete lacZ gene.
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Kit includes: instructions, Bactobeads™, plasmid DNA, buffer, media, ampicillin, X-Gal, ReadyPour™ agar, petri dishes, sterile pipettes, loops and microtubes.
All you need: 37ºC incubation oven, waterbath, microwave or hot plate.
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