This open-ended laboratory activity allows students to design experiments that will generate specific DNA fragments and determine the accuracy of predicted sizes after separation by agarose gel electrophoresis. Gels are stained with InstaStain® Blue or FlashBlue Stain.
For 6 Lab Groups
Restriction Enzyme Digests 35-60 mon. Electro. 45 min.
Kit includes: instructions, plasmid DNAs, Lambda DNA, Standard DNA Fragments, Dryzymes® - Eco RI and Bam HI, Restriction enzyme dilution and reaction buffers, enzyme grade water, various solutions and buffers, agarose powder, InstaStain® Blue and FlashBlue™ Stain.
You need: electrophoresis apparatus, power supply, automatic pipet with tips, waterbath, balance, microwave or hot plate, , visualization (white light), misc. lab glassware, pipet pumps or bulbs, metric rulers, floating racks, distilled or deionized water, ice.
Storage: Some Components Require Freezer & Refrigerator Storage