Analysis of DNA Methylation Using Restriction Enzymes
In this experiment, students explore the effects of DNA methylation on restriction enzyme activity. Plasmid DNA will be digested with the restriction enzymes DpnI and DpnII. When digested with these enzymes, methylated and unmethylated DNA will produce restriction fragments that are distinct from one another. The restriction fragments are then analyzed using agarose gel electrophoresis. After visualizing the gel, students determine which sample is methylated.
* Requires wet ice shipment for next day delivery (by 3:00 pm in most areas).
Kit includes: instructions, methylated and unmethylated Plasmid DNA, EdvoQuick™ DNA Ladder, DpnI and DpnII Restriction Enzymes, various solutions & buffers, agarose powder, InstaStain® Ethidium Bromide
You need: electrophoresis apparatus, power supply, automatic pipet with tips, waterbath, balance, microwave or hot plate, visualization (white light or UV), misc. labware, pipet pumps or bulbs, metric rulers, floating racks, photodocumentation system (optional), distilled or deionized water, ice.