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Isolation of E. coli Chromosomal DNA 203
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Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA Extraction LyphoCells™ and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analyzed by agarose gel electrophoresis as an optional lab extension activity.
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Kit includes: instructions, LyphoCells™, various solutions and buffers, agarose powder, FlashBlue™ Stain.
You need: waterbath, pipet pumps or bulbs, lab glassware, distilled or deionized water, 95-100% isopropanol. For optional electrophoresis: electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave oven or hot plate, misc. labware, white light visualization system, and photodocumentation system.
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