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Transformation of E.coli with pBR322 201
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Transformation is of central importance in molecular cloning since it allows for the selection, propagation, expression and purification of a gene. Positive selection for cells containing plasmid DNA is accomplished by antibiotic growth selection. In this experiment, your students will transform bacteria with the first plasmid made for genetic engineering in 1970, pBR322.
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Kit includes: instructions, Bactobeads™, plasmid DNA, buffer, ampicillin, calcium chloride, ReadyPour™ Agar, Luria broth, Petri dishes, sterile pipettes and loops, microtubes.
All you need: 37ºC incubation oven, waterbath, microwave or hot plate.
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