Sickle Cell Anemia is a common genetic disease that causes long rods in red blood cells, giving them a "sickled" appearance. These cells get stuck in small capillaries of the blood stream leading to oxygen deprivation that causes pain and organ damage. Sickle Cell Anemia is caused by a single point mutation in the hemoglobin gene that results in a faulty protein. In this experiment, your students will investigate the restriction enzyme that discriminates between HbA (normal) and HbS (disease) genes and perform a simulated test on a patient.
Kit includes: instructions, Ready-to-Load™ QuickStrip™ DNA samples, UltraSpec-Agarose™ powder, practice gel loading solution, electrophoresis buffer, InstaStain® Blue and FlashBlue™ stain, calibrated pipet, and microtipped transfer pipets.
All you need: electrophoresis apparatus, power supply, automatic micropipet and tips, balance, microwave or hot plate, visualization system.
Storage: Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.